ERα系列抗體的應(yīng)用研究（雌激素受體研究值得關(guān)注）

引言：  雌激素受體alpha (ERα)分布于子宮、睪丸、腦下垂體、腎、附睪、腎上腺、乳腺等組織，其作為核轉(zhuǎn)錄因子參與人體多種復(fù)雜生理過(guò)程的調(diào)節(jié)。ERα受體表達(dá)和調(diào)節(jié)的研究對(duì)多種疾病的預(yù)防和治療有積極意義，例如癌癥、代謝性疾病、心血管疾病、神經(jīng)退行性疾病、炎癥和骨質(zhì)疏松癥等[1]。蛋白表達(dá)在ERα受體表達(dá)調(diào)節(jié)研究中占有重要地位，而ERα抗體則是ERα受體表達(dá)的重要研究工具。 

       雌激素受體alpha (ERα)分布于子宮、睪丸、腦下垂體、腎、附睪、腎上腺、乳腺、骨等組織，其作為核轉(zhuǎn)錄因子參與人體多種復(fù)雜生理過(guò)程的調(diào)節(jié)。ERα受體表達(dá)和調(diào)節(jié)的研究對(duì)多種疾病的預(yù)防和治療有積極意義，例如癌癥、代謝性疾病、心血管疾病、神經(jīng)退行性疾病、炎癥和骨質(zhì)疏松癥等[1]。蛋白表達(dá)在ERα受體表達(dá)調(diào)節(jié)研究中占有重要地位，而ERα抗體則是ERα受體表達(dá)的重要研究工具。 

      ERα受體包括多個(gè)功能結(jié)構(gòu)域，不同的轉(zhuǎn)錄剪接產(chǎn)生不同的轉(zhuǎn)錄異構(gòu)體[2],主要包括ERα36和ERα46。由于與疾病的密切相關(guān)性，ERα36、ERα46和野生型ERα (ERα66)一起成為ER家族研究熱點(diǎn)，相應(yīng)的抗體也成為對(duì)應(yīng)的研究工具。美國(guó)CHI Scientific提供的 ERα系列（ER-α36, ER-α46 & 66, ER-α36）抗體靈敏度高，特異性強(qiáng)，自面試以來(lái)，引用該系列產(chǎn)品的文獻(xiàn)達(dá)上百篇。尤其是Anti-hER-α36抗體，為美國(guó)CHI Scientific生產(chǎn)銷售的產(chǎn)品，為廣大中外科研學(xué)者研究ERα36相關(guān)疾病提供強(qiáng)有力支撐。

論文截圖：
 

Anti-hER-α36：

The antibody was raised against a synthetic peptide antigen corresponding to the unique C-terminal 20 amino acids of hER-α36. The specificity of the antibody was tested in hER-α36 expression vector transfected HEK293 cells that do not express endogenous hER-α. Immunofluorescence was used to demonstrate immunoreactive signals only in transfectants with the hER-α36-expressing vectors but not in transfectants harboring an empty expression vector. This rabbit polyclonal anti-hER-α36 antibody is supplied in affinity-purified form.

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Anti-hER-α46 & ER-α66:

The antibody was raised against a synthetic peptide antigen corresponding to the unique C-terminal 18 amino acids of hER-α66. The specificity of the antibody was tested in hER-α46 and hER-α66 expression cells, MCF-7 Cells and breast carcinoma tissues. This rabbit polyclonal anti-hER-α46 & hER-α66 antibody is supplied in affinity-purified form.

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Anti-hER-α66：

 The antibody was raised against a synthetic peptide antigen corresponding to the unique C-terminal 18 amino acids of hER-α66. The specificity of the antibody was tested in hER-α66 expression cells, MCF-7 Cells and breast carcinoma tissues. This rabbit polyclonal anti-hER-α66 antibody is supplied in affinity-purified form.

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